Both teams will rate their chances of reaching the Champions League quarter-finals with a welcome draw as the knockout stages begin.
The Serie A side scrapped through after a stunning comeback from three defeats to reach this stage, and will find themselves as favourites despite Valencia's superior experience at this level.
Los Che topped a group which contained Chelsea and Ajax and may relish a return to European competition amid an inconsistent domestic campaign.
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Atalanta come into the tie with a fully-fit squad, with Chelsea loanee Mario Pasalic pushing for a start.
Valencia have far more troubles, with Gabriel Paulista and Ezequiel Garay ruled out in defence. Eliaquim Mangala should come in.
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