When asked if he would not seek an extension even if it is set out in law that he must, Mr Johnson said: “I will not. I don’t want a delay.”
The comments come a day after “he said he would rather be 'dead in a ditch” than delay the UK's departure from the bloc for a third time.
The Prime Minister lost two key votes in the Commons after MPs voted to block no deal, before then rejecting an early general election.
The legislation to delay Brexit rather than leave without a deal - a bill the Prime Minister said would "scupper negotiations" - passed all stages in the Commons on Wednesday.
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But controversially, the Prime Minister culled 21 Conservative MPs who voted against the whip.
Meanwhile, MPs will return to Parliament for the Queen's speech on October 14.
Remain supporting ministers could oust Johnson in a vote of no confidence if he indicates he will refuse to delay Brexit.
But speaking traveling to Scotland to meet the Queen at Balmoral on Friday, a defiant Prime Minister declined to rule out resigning if he failed to deliver Brexit by October 31.
He said: “That is not a hypothesis I'm willing to contemplate. I want us to get this thing done.”