Veteran captain Emilio Nsue led from the front as Nzalang Nacional continued their fantastic record of reaching the knockouts in all four of their appearances at AFCON.
Having topped a group containing Nigeria and hosts Ivory Coast, they will be looking to push into the latter stages this afternoon despite a tough tie against either Egypt or DR Congo awaiting the victor in the quarter-finals.
Guinea are out to end their curse of losing all six knockout games they have played at the Cup of Nations, despite finishing second in 1976 - when the final round was a second group stage.
A draw with Cameroon and victory over The Gambia were enough for them to qualify as a third-placed side for this stage, pipped to second spot in Group C at the last after defeat by holders Senegal.
Date, kick-off time and venue
Equatorial Guinea vs Guinea is scheduled for a 5pm GMT kick-off today on Sunday January 28, 2024.
The match will take place at Alassane Ouattara Stadium in Abidjan, Ivory Coast.
Where to watch Equatorial Guinea vs Guinea
TV channel: In the UK, the game will be televised live on Sky Sports Football, with coverage starting at 4.55pm.
Live stream: Subscribers can also catch the contest live online via the Sky Go app.
Live blog: You can follow all the action on matchday via Standard Sport’s live blog.
Equatorial Guinea vs Guinea team news
Head coach Juan Micha has stuck with a consistent selection for Equatorial Guinea's three games so far and will surely opt for the same 4-3-3 setup for this tie.
Top scorer Nsue will continue up front after his brace in the stunning 4-0 win over Ivory Coast put him on five goals as the leading marksman at this AFCON so far.
In-form Stuttgart striker Serhou Guirassy managed an hour on his return from injury for Guinea's defeat by Senegal, so he should once again get the nod in Abidjan.
Captain Naby Keita also started against Senegal after missing the games against Cameroon and The Gambia, while Francois Kamano is also back after suspension.
Equatorial Guinea vs Guinea prediction
That this game is taking place in the centrepiece stadium for Ivory Coast's tournament underlines just how much of a shock it is that Equatorial Guinea topped the hosts' group.
They may lack the couple of star names of their opponents but Nsue's superb form could be the difference on what is turning into a historic run.
Equatorial Guinea to win, 2-0.
Head to head (h2h) history and results
This is the first listed meeting between these two teams.
Equatorial Guinea vs Guinea match odds
Equatorial Guinea to win: 14/5
Guinea to win: 6/5
Draw (90 mins): 9/5
Odds via Betfair (subject to change).