The England striker was not involved in the team that beat Newport in the FA Cup at the weekend, having missed training through illness after a well-publicised night out in Belfast.
Rashford, 26, was spotted returning to Carrington on Monday after United boss Erik ten Hag vowed to deal with the situation as an "internal matter".
On Monday night, the club released a statement revealing that Rashford had "taken responsibility for his actions" with the matter now resolved, with Ten Hag echoing that sentiment at his pre-Wolves press conference on Wednesday afternoon.
Antony replaced the United academy product in south Wales and scored the winning goal, meaning that Rashford has won to start ahead of the Brazilian at Molineux tonight.
Mason Mount, Anthony Martial, Victor Lindelof and Tyrell Malacia are still absent through injury.
Aaron Wan-Bissaka is also out after sitting out the Newport trip, while Andre Onana is back in goal after his disastrous Africa Cup of Nations campaign with Cameroon ended.
However, Sofyan Amrabat is not back in time after pre-tournament favourites Morocco fell in the AFCON last-16 on Tuesday evening.
Man Utd XI: Onana; Dalot, Varane, Martinez, Shaw; Mainoo, Casemiro; Garnacho, Fernandes, Rashford; Hojlund
Subs: Bayindir, Maguire, Eriksen, Diallo, Antony, Evans, McTominay, Kambwala, Forson
Injuries: Martial, Lindelof, Mount, Malacia, Wan-Bissaka
Time and date: 8:15pm GMT on Thursday February 1, 2024
TV channel: TNT Sports 1